Rats fasted overnight before surgery. Subdiaphragmatic vagotomy and pyloroplasty were carried out in 15 rats, under isoflurane anesthesia. The stomach and lower esophagus were exposed after upper midline laparotomy. The stomach was gently retracted down beneath the diaphragm and, under a microscope, anterior and posterior vagal trunks were visualized and isolated from the esophagus about 2 cm above the stomach. Two sutures were tied around each vagal trunk with sterile 4-0 silk. The stomach was repositioned to its original location, and the muscular abdominal wall and skin were successively closed with sterile 4-0 silk I Didn’t Plan On Becoming A Grouchy Demanding Asshole Mom Who Yells A Lot But Here I Am Shirt suture. In sham-operated rats, a similar abdominal incision was made.
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The stomach was retracted, vagal trunks were identified and manipulated, and pyloroplasty was performed. Four out of 15 rats died shortly after the procedure due to complications. The remaining 11 rats survived and maintained good health. All operated rats were allowed 1 wk to recover prior to any experimental procedure. During that period, all animals were handled each day to familiarize them with the injection procedure. During the first four postoperative days, rats gained less body weight than sham-operated animals but returned to their preoperative daily body weight gain. At the end of the recovery period, no I Didn’t Plan On Becoming A Grouchy Demanding Asshole Mom Who Yells A Lot But Here I Am Shirt difference in body weight gains was detected between and sham-operated animals.